Composite

Part:BBa_K1973028:Design

Designed by: José Martín Gómez   Group: iGEM16_UPO-Sevilla   (2016-10-14)


miniTn7BBGm-nahR-Psal-nasF-glpF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4367
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4367
    Illegal BglII site found at 3051
    Illegal BglII site found at 3322
    Illegal BglII site found at 3608
    Illegal BglII site found at 4972
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4367
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4367
    Illegal XbaI site found at 4382
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 4804
    Illegal NgoMIV site found at 5685
    Illegal NgoMIV site found at 5760
    Illegal NgoMIV site found at 5817
    Illegal NgoMIV site found at 6231
    Illegal NgoMIV site found at 6324
    Illegal AgeI site found at 5658
    Illegal AgeI site found at 6174
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal SapI.rc site found at 2763


Design Notes

This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973026 together.


Source

This composite part was created by cloning the parts BBa_K510000, BBa_K1973033, BBa_K1973001 and BBa_K1973026 together.

References